Cytokine production by splenocytes stimulated with inactivated bacteria of different <i>Bifidobacterium</i> strains.

<p>Splenocytes isolated from naïve mice (n = 5) were stimulated with formalin-inactivated bifidobacteria (6 x 10⁷ CFU/ml) for 48 h. Pam3CSK4 (PAM3C, 1μg/ml) was use as a positive control. Non-stimulated splenocytes (Medium) were evaluated as control of basal cytokine levels. Concentration of cytokines in supernatants was determined by multiplex assay. Data are expressed as mean ± SEM. Results are representatives of two repeat experiments. Significant difference to medium was calculated using One-way ANOVA and Dunnett’s multiple comparison post-hoc test *p < 0.05; **p < 0.01.</p><p>Cytokine production by splenocytes stimulated with inactivated bacteria of different <i>Bifidobacterium</i> strains.