Fragmentation of Gag p24 broadens the T-cell repertoire.

<p>HLA-A2/DR-transgenic mice were immunized three times with either the full set of overlapping peptides (OLGa, 2 µg/peptide) with or without CAF05 or protein (5 µg) in CAF05. Naïve mice where used as the negative control. 10 days after the last immunization, splenocytes from individual mice were restimulated with the indicated peptide pool (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0063575#pone-0063575-t001" target="_blank">Table 1</a>) and the frequency of IFN-γ-producing cells was determined by ELISPOT. Shown are values after background subtraction. Black symbols indicate a significant response for that individual mice as determined by the DFR(x2) method. If a significant response was detected in either the protein/CAF05 or peptides/CAF05 group, statistically significant differences in SFU-levels between these two groups only were determined based on a one-tailed t test and <i>P</i>-values are indicated. NS, not significant.