Immunization with the four double restricted epitopes induces both CTL and Th2 reactivity.

<p>Five groups of HLA transgenic mice were immunized intraperitoneally three times at two week intervals with CAF09 plus one of the four double restricted peptides or M(58–66) (20nM) [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0145629#pone.0145629.ref021" target="_blank">21</a>]. One week after last immunization, IFNγ (A) or IL-4 (B) ELISpot was used to measure spot forming units (SFU) from splenocytes. Cells were pooled from each group and depleted for CD4 or CD8 positive cells before being stimulated <i>ex vivo</i> with peptide in four replicate cultures. Each bar represents mean ± SD of IFNγ (A) or IL-4 (B) spot forming units (SFU) of groups of 4 immunized mice. Experiments were conducted twice.