GFP and Gag-Specific immune responses induced by rBCG vaccines.

<p>Mice were vaccinated with BCG[pHS207], [pWB105], [pWB106] and [pWB206] (intranasal, 10⁷ CFU). Splenocytes pooled from a group of 5 mice were used on day 56 in A. an IFN-γ ELISPOT assay with the GFPCD8 peptide, GagCD8 peptide or GagCD4 peptide. Bars represent the average number of SFU/10⁶ splenocytes ± the standard deviation of triplicate reactions after subtraction of average background responses of not more than 20±10 SFU per 10⁶ splenocytes. No response to the GagCD8 peptide was detected. B. Splenocytes were cultured (48 h) with the GFPCD8 peptide, GagCD8 peptide or GagCD4 peptide and cytokine levels in the culture supernatant were quantified using a Th1/Th2 cytokine bead array assay and flow cytometry. Only IFN-γ was detected in the culture fluid and for splencoytes stimulated with the GFPCD8 and GagCD4 peptides. Levels are expressed as pg/10⁶ splenocytes and are from a representative experiment.