Influenza infected HLA transgenic mice show no reactivity towards any of the immunogenic peptides.

<p>HLA transgenic mice were challenged intranasally with 4 LD₅₀ (B) or 0.4 LD₅₀ (A and C) of influenza A. IFNγ production from splenocytes was detected 10 days later using IFNγ ELISpot (A and B) or flow cytometry (C). In A and B, each bar represents mean ± SD of IFNγ spot forming units (SFU) of groups of 4–5 mice from which splenocytes were pooled before being stimulated <i>ex vivo</i> with peptide in four replicate cultures. In C, each bar represents mean ± SD of the number of IFNγ producing splenic CD8 T cells from 2 individual mice after <i>ex vivo</i> stimulation with peptide. Experiments were conducted 1–4 times depending of the peptide. Experiments were conducted 1–4 times, depending on the peptide.