Gcnf mutants undergo meiosis normally.

<p>(A–D) Immunofluorescence for SYCP3 (red) and GCNA (green) in <i>Gcnf^+/+TAM</i> wild-type (A,B) and <i>Gcnf^fl/fl;Ubc-Cre-ERT2^TAM</i> mutant (C,D) ovaries at E16.5; insets show higher magnification of areas boxed in white, SYCP3 staining alone. 100% of GCNA+ cells in both wild type (60/60) and mutant (84/84) were also SYCP3+. B and D, Deconvolution microscopy images demonstrating thread-like staining of SYCP3. Single-channel images showing SYCP3 alone are shown to the right of two-channel images. (E and F) Immunofluorescence for γH2AX (red) and GCNA (green) in <i>Gcnf^+/+TAM</i> wild-type and <i>Gcnf^fl/fl;Ubc-Cre-ERT2^TAM</i> mutant ovaries at E16.5. Single-channel images are shown to the right. Anterior is to the left. Scale bars, 50 um (A,C,E,F) or 5 um (B,D).