Follicle cell microvilli display an apparently normal morphology in <i>Sans</i> and <i>ck</i> mutant flies.

<p>A–C: Cross-sectional views of (A) wild-type, (B) <i>ck¹³/ck¹³</i> mutant, and (C) <i>Sans²⁴⁵/Sans²⁴⁵</i> mutant follicle cells of stage 10-egg chambers stained for F-actin (red), Cad99C (green), and α-Tubulin (blue). At this level of resolution, microvilli appear to be normal in the mutants. D: Clones of <i>Sans²⁴⁵/Sans²⁴⁵</i> mutant follicle cells marked by the absence of GFP (blue) of stage 10-egg chambers stained for F-actin (red) and Cad99C (green). A cross-sectional view is shown (apical is to the top). D′ displays a heat-map of the staining shown in D. Cad99C is reduced on microvilli of <i>Sans²⁴⁵/Sans²⁴⁵</i> mutant follicle cells. E: Western blot analysis of ovaries with an anti-Cad99C antiserum. Genotypes of the flies are indicated. Similar amounts of Cad99C (arrow) are detected in extracts of wild-type (lane 1), heterozygous (lanes 3,5,7), and homozygous (lanes 2,4,6,8) <i>Sans</i> mutant ovaries. Subsequent blotting with an anti α-Tubulin antibody (lower panel) shows the loading of similar amounts of proteins in each lane. Molecular weights are indicated to the left (in kDa). Scale bars: A–C: 10 µm; D: 20 µm.