<i>Gcnf</i> expression in fetal ovarian germ cells.

<p>(A) Levels of <i>Gcnf</i> transcript in female (XX) and male (XY) gonads from wild-type and germ-cell-depleted (<i>W/W^v</i>) mutant embryos at E12.5, E14.5, and E16.5, as determined by Illumina sequencing of gonadal RNA. Plotted here are average numbers of reads of <i>Gcnf</i> per million total reads from two individual biological replicates. (B) Single molecule fluorescence in situ hybridization for <i>Gcnf</i> mRNA (red) in sections of XX genital ridges or gonads, with germ cells marked by SSEA1 (green), and nuclei marked by DAPI staining (blue). Large red-orange spots in the E14.5 image are auto-fluorescent blood cells. Boxes indicate areas shown in higher magnification below each image. Scale bar, 20 um.