SC central element assembly defects in <i>csn</i> mutants.

<p>A) CSN alleles used in this study: black rectangles represent exons, black lines introns, gray areas represent UTR regions, red lines region deleted and purple, green and blue rectangles the protein domains. PAM and PINT are subdomains of the PCI domain, B) Micrographs of SYP-1 (red or grey scaled) and DAPI (blue) stained wild-type and <i>csn-5</i> mutants nuclei representing the various stages of the <i>C. elegans</i> gonad. Images are projections through half of a three-dimensional data stacks. Scale bar is 2 µm. PMT = pre-meiotic tip, TZ = transition zone, EP = early pachytene, MP = mid pachytene, LP = late pachytene. SYP-1 aggregates appear in the TZ-like stage of the gonad and persist through the LP-like stage. C) Whole gonad from wild-type and <i>csn</i> mutants SYP-1 and DAPI stained. Images show smaller gonads in <i>csn</i> mutants and lack of oocytes progressing through diakinesis. Scale Bar 16 µm. SYP-1 (grayscale) staining only of gonads showing aggregation throughout the gonad, starting at transition zone.