Antibody responses after multiple immunizations.

<p>C57/BL6 mice were immunized two times with OVA protein alone or mixed with 10⁶ IU VREP-FliC-D3. Control mice were given PBS. Each immunized group consisted of five mice, and two control mice were used. Serum was assayed for anti-OVA IgG by ELISA after one (black bars) or two (gray bars) immunizations. A Student’s <i>t</i>-test was used to compare the response after one and two administrations of the same vaccine. * <i>P</i><0.05.