TA localization in the mouse cerebellar cortex at P14.

<p><b>A-A</b>’’and <b>B–B</b>’<b>’)</b> Cerebellar sagittal section immunostained for PV (green) and TA (red) and counterstained with Dapi (cyan). <b>A</b>) Merge image between Dapi-positive nuclei (cyan) and PV-positive PC dendrites (green) in the upper part of the ML and the EGL. <b>A</b>’) Shows the corresponding TA immunostaining (red). <b>A</b>’’<b>)</b> Merge image of all markers (PV-TA-Dapi) plus the colocalization mask TA/PV (mTA/PV in white) highlighting the expression of TA in PC dendrites and spines. <b>B</b>) Merge image of PV staining and Dapi-positive nuclei, highlighting the PV-positive PC bodies and dendrites and interneurons of the ML (green). The inset (white box) is an high magnification of a BC body. <b>B</b>’) TA protein (red) is abundantly present in PCs and BCs (inset), both in the cytoplasm and nuclei. Black spaces are TA-negative nuclei in the ML. <b>B</b>’’) merge image of all markers (PV-TA-Dapi) plus the colocalization mask TA/PV (mTA/PV in white). <b>C</b>) Merge image between DAPI-positive nuclei and V1-positive (green) rosettes to define the IGL. Most of the DAPI-positive nuclei in the IGL belong to granule cells <b>C</b>’) TA staining is diffuse also in this layer; granule cells (high magnification in the inset) and Golgi-like cells are intensively labeled. <b>C</b>’’) Merge image of all markers (TA-V1-Dapi) plus the colocalization mask TA/Dapi (mTA/Dapi in white). The absence of the white mask highlights no expression of TA in almost all cell nuclei. Few white dots in the inset indicate overlapping signal of TA around the nucleus. Scale bars in A, B and C 10 µm; in the insets 2 µm.