TA is localized in V2-positive CFs, VGAT-positive BC and PC terminals at P14.

<p>Merge of TA (red) and V2 (cyan) staining and of the colocalization mask TA/V2 (mTA/V2 white). TA is localized in the V2-positive CF terminals which impinge on the PC proximal dendrite. <b>B</b>) The negative CTR (CTR-) is represented by the overlapping signal between the V2-positive CFs (cyan) contacting the Cb-positive PC dendrites (green). The panel on the right is the relative colocalization mask (mCb/V2, white). <b>C</b>) Quantitative colocalization analysis of TA in V2-positive terminals. The mean of the TA/V2 overlap coefficients was significantly different from the negative CTR mean value. The insets are high magnification of the white boxes in A and B and show V2 and the relative colocalization masks. <b>D</b>) Merge of TA (red) and VGAT (cyan) staining and of the colocalization mask TA/VGAT (mTA/VGAT white) in the PCL and ML. TA is localized in the VGAT-positive terminals of inhibitory neurons contacting PC-dendrites and bodies. <b>E</b>) The negative CTR (CTR-) is represented by the overlapping signal between the VGAT-positive terminals (cyan) and the Cb-positive PC bodies (green). VGAT-positive PC collaterals were excluded from the analysis. The panel on the right is the relative colocalization mask (mCb/VGAT, white). <b>F</b>) Quantitative colocalization analysis of TA in VGAT-positive terminals (gray column). The mean of the TA/VGAT overlap coefficients was significantly different from negative CTR mean value (white column). The insets are high magnification of the white boxes in D and E showing VGAT and the relative colocalization masks. <b>G</b>) Merge of TA (red) and VGAT (cyan) staining and of the colocalization mask TA/VGAT (mTA/VGAT DCN, white) in the DCN region. TA is localized in the VGAT-positive synaptic terminals of PCs which contact DCNs. <b>H</b>) The relative negative control was the overlapping signal of VGAT-positive terminals (cyan) impinging on SMI32-positive DCN bodies (green). The right panel is the relative colocalization mask (m SMI32/VGAT DCN, white). <b>I</b>) Quantitative colocalization analysis of TA in VGAT-positive terminals (gray column). The mean of the TA/VGAT overlap coefficients was significantly different from negative CTR mean value (white column). The insets are high magnification of the white boxes in G and H and show VGAT and the relative colocalization masks. *** p < 0.001. Data are represented as mean± SEM. Scale bars in A-B, D-E and G-H: 10 µm; in C–F–I: 1 µm.