Colocalization of TH and GAD-67 immuonoreactivity with FG-labeled cells in the VTA.

<p>[A] Representative composite photomicrograph of the middle level of the VTA displaying the overlay of FG (blue), TH-IR (green) and GAD-67-IR (red). Inset displays the schematic diagram of the middle VTA (shaded area) and surrounding brain areas (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0020508#pone-0020508-g003" target="_blank">Fig. 3</a> for abbreviations] at bregma -5.64 mm. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0020508#pone.0020508-Paxinos1" target="_blank">[17]</a>. High magnification images of the boxed region in panel A depict FG labeling [blue, B], TH-IR [green, C], and GAD-67-IR [red, D], as well as the overlay of images in B, C, and D to demonstrate colocalization [E]. Filled arrows (<b></b>) indicate a cell triple-labeled for FG+TH+GAD-67, open arrows (<b></b>) indicate a cell double-labeled for FG+TH, solid arrows () indicate a cell double-labeled for FG+GAD-67, and the arrowheads () point to a cell double-labeled for TH+GAD-67 in the absence of FG; Scale bars = 20 µm. Note: Portions of IP nucleus present in the composite photomicrograph in panel A were removed from the image prior to incorporation into the figure.