Colocalization of TH and 5-HT_2CR immuonoreactivity with FG-labeled cells in the VTA.

<p>[A] Representative composite photomicrograph of the middle level of the VTA displaying the overlay of FG (blue), TH-IR (red) and 5-HT_2CR-IR (green). Inset displays the schematic diagram of the middle VTA (shaded area) and surrounding brain areas [interpeduncular nucleus (IP); medial laminiscus (ml); mammillary peduncle (mp); substantia nigra pars compacta, dorsal tier (SNCD); substantial nigra reticulata (SNR)] at bregma −5.64 mm <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0020508#pone.0020508-Paxinos1" target="_blank">[17]</a>. High magnification images of the boxed region in panel A depict FG labeling [blue; B], TH-IR [red, C], and 5-HT_2CR-IR [green, D], as well as the overlay of images in B, C and D to demonstrate colocalization [E]. Filled arrows (<b></b>) indicate cells triple-labeled for FG+TH+5-HT_2CR, while open arrows (<b></b>) indicate a cell double-labeled for FG+TH; Scale bars = 20 µm. Note: Portions of IP nucleus present in the composite photomicrograph in panel A were removed from the image prior to incorporation into the figure.