(A) Schematic diagrams of a coronal image showing the brain areas (boxed) used for analysis (left) and a sagittal image depicting the level (dashed line) where each coronal section was obtained (right). (B, C) Brain sections of Brn3bGFP/+ mouse labeled with anti-GFP (i.e., Brn3b; green) and in situ probe to Tac2 (red) at P18. (B) (Left) No Tac2+ signals were detected in the superficial and intermediate SC layers. (Right) Magnified view of the boxed areas (i, ii). (C) (Left) Tac2+ cells are detectable in deep SC/PAG. (Right) Magnified view of the boxed areas (iii, iv). Quantification revealed that approximately 57% of Tac2+ cells are Brn3b+ (70.7 ± 7.2 cells/animal, n = 3 animals). Arrows indicate examples of overlapping signals. DAPI (blue)...
<p>Immunohistochemistry on free floating adult mouse brain sections using the polyclonal B<sup>0</su...
<p>Confocal images of coronal brain sections showing non-neuronal GFP+ cells in several locations. (...
(A) Schematic diagrams showing the brain area (boxed) of a coronal section used for analysis (top) a...
(A, B) Transcriptomic profiling (RNA-seq) using dorsal midbrain at P2-4 (n = 3 for control and 4 for...
(A) Schematic diagram of Tac2 + mCherry delivery into the deep SC/PAG layer. AAV was used as a deliv...
(A) Schematic diagram of a coronal image showing the brain areas (boxed) used for analysis (left) an...
(A) CRISPR/Cas9-mediated genome-editing strategy to generate Brn3bGFP/+ (Brn3b-GFP) knock-in mouse l...
<p><b>A-A</b>’’and <b>B–B</b>’<b>’)</b> Cerebellar sagittal section immunostained for PV (green) and...
<p>Fluorescence images of sections of sensory ganglia and regions of the brain illustrate the cellul...
(A) Schematic diagram of AAV-mCherry and AAV-Tac2 + AAV-mCherry delivery into the deep SC/PAG of WT ...
<p>Immunohistochemistry on free floating adult mouse brain sections using the polyclonal B<sup>0</su...
(A–C) Schematic diagrams of coronal images showing the brain area (boxed) used for analysis (left) a...
<p>(a) Immunohistochemisty showing expression overlap between GFP and genes expressed by propriocept...
<p>(A–D) Results of immunohistochemical staining with a KCC2 - specific antibody (red) in SNr GABAer...
<p>A) Confocal fluorescence images of the same horizontal section of the ventral entorhinal cortex o...
<p>Immunohistochemistry on free floating adult mouse brain sections using the polyclonal B<sup>0</su...
<p>Confocal images of coronal brain sections showing non-neuronal GFP+ cells in several locations. (...
(A) Schematic diagrams showing the brain area (boxed) of a coronal section used for analysis (top) a...
(A, B) Transcriptomic profiling (RNA-seq) using dorsal midbrain at P2-4 (n = 3 for control and 4 for...
(A) Schematic diagram of Tac2 + mCherry delivery into the deep SC/PAG layer. AAV was used as a deliv...
(A) Schematic diagram of a coronal image showing the brain areas (boxed) used for analysis (left) an...
(A) CRISPR/Cas9-mediated genome-editing strategy to generate Brn3bGFP/+ (Brn3b-GFP) knock-in mouse l...
<p><b>A-A</b>’’and <b>B–B</b>’<b>’)</b> Cerebellar sagittal section immunostained for PV (green) and...
<p>Fluorescence images of sections of sensory ganglia and regions of the brain illustrate the cellul...
(A) Schematic diagram of AAV-mCherry and AAV-Tac2 + AAV-mCherry delivery into the deep SC/PAG of WT ...
<p>Immunohistochemistry on free floating adult mouse brain sections using the polyclonal B<sup>0</su...
(A–C) Schematic diagrams of coronal images showing the brain area (boxed) used for analysis (left) a...
<p>(a) Immunohistochemisty showing expression overlap between GFP and genes expressed by propriocept...
<p>(A–D) Results of immunohistochemical staining with a KCC2 - specific antibody (red) in SNr GABAer...
<p>A) Confocal fluorescence images of the same horizontal section of the ventral entorhinal cortex o...
<p>Immunohistochemistry on free floating adult mouse brain sections using the polyclonal B<sup>0</su...
<p>Confocal images of coronal brain sections showing non-neuronal GFP+ cells in several locations. (...
(A) Schematic diagrams showing the brain area (boxed) of a coronal section used for analysis (top) a...