Effect of HIFs or ETS knockdown on CD133 promoter activity and expression in WiDr cells.

<p>(A) P5 −98 bp promoter activity under knockdown of HIF-1α and HIF-2α. (B) P5 −98 bp promoter activity under knockdown of ETS families. (C) P5 −98 bp promoter activity after overexpression of HIF-1α and HIF-2α 24 hrs after knockdown of HIF-1α and HIF-2α under normoxia and hypoxia. (D) Quantitative real-time reverse-transcription PCR (qRT-PCR) analysis of CD133 under knockdown of HIF-1α and HIF-2α. *<i>P</i><0.05 vs. control siRNA (siCont). (E) Western blot analysis of HIF-1α and HIF-2α and CD133 under knockdown of HIF-1α and HIF-2α. β-actin is an internal control.