Influence of HIF–1α knockdown by siRNA in ASCs under hypoxia (A) The relative mRNA expression levels of HIF–1α in ASCs after transfection for 48 h under hypoxia. HIF–1α level was suppressed significantly compared with that in the control. (B) The relative mRNA expression levels of FGF–2 in ASCs after transfection for 48 h under hypoxia. FGF–2 level was suppressed significantly compared with that in the control. (C) The relative mRNA expression levels of VEGF in ASCs after transfection for 48 h under hypoxia. VEGF level was suppressed significantly compared with that in the control. Data are the means ± SD. *p < 0.05. (D) Expression of HIF–1α was inhibited by siRNA using western blotting.

<p>Influence of HIF–1α knockdown by siRNA in ASCs under hypoxia (A) The relative mRNA expression levels of HIF–1α in ASCs after transfection for 48 h under hypoxia. HIF–1α level was suppressed significantly compared with that in the control. (B) The relative mRNA expression levels of FGF–2 in ASCs after transfection for 48 h under hypoxia. FGF–2 level was suppressed significantly compared with that in the control. (C) The relative mRNA expression levels of VEGF in ASCs after transfection for 48 h under hypoxia. VEGF level was suppressed significantly compared with that in the control. Data are the means ± SD. *p < 0.05. (D) Expression of HIF–1α was inhibited by siRNA using western blotting.