<p>(<b>A</b>) The expression of HIF-1α and CX3CR1 in five PDAC cells assessed by Western-blotting. (<b>B</b>) HIF-1α and CX3CR1 expression determined by Western-blotting (upper) and qRT-PCR (lower) on MiaPaCa2 (left) and Patu8988 (right) cells cultured under normoxia (21% O<sub>2</sub>) or hypoxia (1.5% O<sub>2</sub>) for different times. *P<0.05 versus control. (<b>C</b>) Three specific siRNAs targeting HIF-1α (left) (50 nM) and HIF-2α (right) (50 nM) on MiaPaCa2 cells. (<b>D</b>) Western-blotting for evaluating the expression of CX3CR1 after knockdown of siHIF1α #2 (50 nM) and siHIF2α #2 (50 nM) in MiaPaCa2 cells under normoxia and hypoxia conditions.</p
<p>A: Real-time RT-PCR analysis on HIF-1α (<i>a</i>), HIF-2α (<i>b</i>) and CTR1 (<i>c</i>) in hypox...
<p><b>(A-C):</b> siRNA mediated knockdown of HIF-1α reversed hypoxic downregulation of MHC class I e...
<p>(A) After the transfection of HIF1A siRNAs, AsPC1 cells were cultured under normoxia (N) or hypox...
<p>MSC were transiently transfected with scrambled sequences (Mock, white bars, open circles) or HIF...
<p>A) HIF-1α expression at different times (0–24 h) after setup of hypoxia; 3 h+3 h: incubation of c...
<p>SOSP-9607 cells were transfected with siHIF-1α or NC-siRNA- and then exposed to both hypoxia and ...
<p>A: CMECs were incubated with siRNA directed against HIF-1α (HIF-1α siRNA) or scrambled oligonucle...
<p>(<b>A</b>) Western blots of whole cell lysates for HIF-1α, HIF-2α and CXCR4 expression under hypo...
<p>(<b>a</b>) HIF1A gene expression of human MSCs obtained by real-time PCR (n = 6; unpaired t-test)...
<p>Western blot analysis of changes in HIF-1α, LDH-M, and PDHK1 expression in whole cell lysate from...
<p>(A) Performed under hypoxic condition (1% O<sub>2</sub>), knockdown of HIF-1α in U937 and HL-60 c...
<p>(A, B): Expression of HIF-1α and SCF protein after hypoxia treatment. Two pancreatic cancer cell ...
<p>Human SOSP-9607 osteosarcoma cells were exposed to either hypoxia or normoxia. <b>A.</b> At the t...
<p>Protein expression of PPAR-γ and HIFs (A) as well as pluripotency marker proteins (B) was examine...
<p>(<b>A</b>) MiaPaCa2 (upper) and Patu8988 (lower) cells were transfected with pcDNA3.1-HIF1α plasm...
<p>A: Real-time RT-PCR analysis on HIF-1α (<i>a</i>), HIF-2α (<i>b</i>) and CTR1 (<i>c</i>) in hypox...
<p><b>(A-C):</b> siRNA mediated knockdown of HIF-1α reversed hypoxic downregulation of MHC class I e...
<p>(A) After the transfection of HIF1A siRNAs, AsPC1 cells were cultured under normoxia (N) or hypox...
<p>MSC were transiently transfected with scrambled sequences (Mock, white bars, open circles) or HIF...
<p>A) HIF-1α expression at different times (0–24 h) after setup of hypoxia; 3 h+3 h: incubation of c...
<p>SOSP-9607 cells were transfected with siHIF-1α or NC-siRNA- and then exposed to both hypoxia and ...
<p>A: CMECs were incubated with siRNA directed against HIF-1α (HIF-1α siRNA) or scrambled oligonucle...
<p>(<b>A</b>) Western blots of whole cell lysates for HIF-1α, HIF-2α and CXCR4 expression under hypo...
<p>(<b>a</b>) HIF1A gene expression of human MSCs obtained by real-time PCR (n = 6; unpaired t-test)...
<p>Western blot analysis of changes in HIF-1α, LDH-M, and PDHK1 expression in whole cell lysate from...
<p>(A) Performed under hypoxic condition (1% O<sub>2</sub>), knockdown of HIF-1α in U937 and HL-60 c...
<p>(A, B): Expression of HIF-1α and SCF protein after hypoxia treatment. Two pancreatic cancer cell ...
<p>Human SOSP-9607 osteosarcoma cells were exposed to either hypoxia or normoxia. <b>A.</b> At the t...
<p>Protein expression of PPAR-γ and HIFs (A) as well as pluripotency marker proteins (B) was examine...
<p>(<b>A</b>) MiaPaCa2 (upper) and Patu8988 (lower) cells were transfected with pcDNA3.1-HIF1α plasm...
<p>A: Real-time RT-PCR analysis on HIF-1α (<i>a</i>), HIF-2α (<i>b</i>) and CTR1 (<i>c</i>) in hypox...
<p><b>(A-C):</b> siRNA mediated knockdown of HIF-1α reversed hypoxic downregulation of MHC class I e...
<p>(A) After the transfection of HIF1A siRNAs, AsPC1 cells were cultured under normoxia (N) or hypox...