The VP/AAP overlapping ORFs in the AAV2<i>cap</i> gene.

<p>(<b>A</b>) Organization of the <i>cap</i> gene and evolutionary conservation of the VP proteins. Evolutionary conservation scores were calculated by a ConSurf analysis <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0066211#pone.0066211-Glaser1" target="_blank">[39]</a> of 128 AAV species. (<b>B</b>) Sequence alignment of amino acids around the VP and AAP overlapping regions indicated with black lines in Panel A. The QVKEVTQ/KSKRSRR motifs are indicated with seven thick vertical lines. The numbers indicate amino acid positions. (<b>C</b>) VP/AAP-overlapping ORFs encoding the QVKEVTQ/KSKRSRR motifs. (<b>D</b>) Amino acid sequence logos (frequency plots) representing the VPs and AAPs derived from 128 AAV species.