Alignment of primary and secondary structures of AnAFP orthologs.

<p>Identified or proposed amino-acid sequences of mature proteins were aligned by CLUSTAL W. Names of corresponding organisms owning the orthologue are abbreviated using the first letter of genus name followed by the first three letters of the species name. Protein/GenBank accession numbers from UniProtKB, AspGD or NCBI, respectively, are indicated in brackets. Amino-acid sequences marked by asterisks were derived from an expressed sequence-tag database analysis. Though some of the listed mature proteins are identical (marked by square brackets), all of them differ at least in one amino acid when considering the pre-pro-regions as well. The overall conserved cysteine residues are highlighted by open boxes. Predicted γ-core motifs are highlighted by shaded boxes. Secondary structures derived from NMR solution structures of AFP [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0165755#pone.0165755.ref070" target="_blank">70</a>] and PAF [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0165755#pone.0165755.ref071" target="_blank">71</a>] and from a secondary structure prediction of AFP, PAF or AnAFP, respectively, are shown at the top. Secondary structure predictions have been calculated using the PredictProtein server [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0165755#pone.0165755.ref066" target="_blank">66</a>] for all mature proteins. Blue arrows and capital E-letters on top depict positions of beta strands whereas lines mean loops or turns, respectively. Gaps are depicted by dots.