DNA synthesis and apoptosis in CD24⁺ cells.

<p>(a,b) Flow cytometric analysis of CD24-gated cells shows that ethanol pre-exposure results in significantly increased incorporation of the nucleic acid analog EdU 24 hours later: (<b>a</b>) depicts sample CD-24 gated histograms of control and ethanol pre-exposed exposed cultures, (<b>b</b>) shows quantification of percentage of EdU-positive cells (M2 gate). (<b>c,d</b>) Activated Caspase assay shows that ethanol pre-exposure does not lead to a significant change in apoptosis 24 hours later: (<b>c</b>) depicts Caspase-activated cells (green fluorescence) with DAPI (blue) as a counter-stain for nuclei. A majority of staurosporine-treated cells were apoptotic (arrows), whereas only an occasional cell was apoptotic in both the control and ethanol-pretreated conditions. (<b>d</b>) Fluorometric quantification shows that control and ethanol-pretreated cells do not exhibit a significant difference in caspase activity, and that both conditions exhibit less apoptosis than staurosporine treatment. Asterisk indicates statistical significance, n.s., not significant.