Effects of ethanol on cell proliferation.

<p>(A, B) Flow cytometry to assess the amount of DNA in the G1, S, and G2 phases during the cell cycle at day 8 after differentiation in the presence or absence of ethanol at 100 mM. (C) qPCR was performed to evaluate the expression of cyclin D1 at days 4 and 8 after differentiation in the presence or absence of ethanol at 100 mM (*p<0.05 vs. no EtOH). (D) Western blot analysis was used to determine protein expression of albumin (ALB), and cyclin D1 at day 8 after differentiation in the presence or absence of ethanol at 100 mM. GAPGH was used as housekeeping gene control.