Ethanol effects on the CD24^depleted population.

<p>Immuno-magnetically selected CD24^depleted subpopulation cultured under mitogenic conditions, re-expresses CD24⁺ cells. (<b>a</b>) Flow cytometric frequency histogram showing the expression of CD24⁺ cells derived from control (left panel) and ethanol pre-treated CD24^depleted cells. ‘X’-axis shows CD24 immunofluorescence intensity and ‘Y’-axis indicates cell number. Bottom panel shows background immunofluorescence following labeling with an isotype-specific antibody. Vertical dashed lines show peak CD24 immunofluorescence in control cells. Sample histograms, and quantitative analysis (<b>b</b>) show variable, but significantly increased numbers of CD24⁺ cells derived from ethanol pretreated, CD24^depleted cells relative to controls. Asterisks indicate statistically significant differences relative to controls.