Blockade of G protein-mediated responses in the recorded neuron prevents the DEX-induced change in mIPSC frequency.

<p><b>A</b>. Continuous recordings recording of mIPSCs after loading of the recorded cell with the G protein inhibitor, GDP _βS (500 µM in the recording pipette). <b>B</b>. mIPSCs recorded in the same neuron after application of DEX (10 µM). <b>A₁</b> and <b>B₁</b> are temporally-expanded, 2 s sections from boxed areas of traces A and B (arrows). <b>C</b>. Cumulative plots of the inter-event interval distribution for mIPSCs in the GDP _βS-loaded cell shown in A and B indicates no effect of DEX (<i>P</i>>0.05; K–S test). <b>D</b>. Graph of mean mIPSC frequency before and after DEX application in GDP _βS-loaded neurons. No effect of DEX was observed in any neuron (n=13; P>0.05).