Adenosine decreases mEPSC frequency with no effects on mEPSC amplitudes.

<p>A, mEPSCs recorded from a layer III pyramidal neuron in the presence of TTX (1 µM) before, during and after application of adenosine (100 µM). <b>B,</b> Time course of the mEPSC frequency averaged from 11 cells. Numbers of mEPSCs at each min were normalized to that of mEPSCs in the 5 min prior to the application of adenosine (n = 11, p<0.001 vs. baseline, paired t-test). <b>C,</b> Cumulative frequency distribution from a layer III pyramidal neuron before (<i>solid</i>) and during (<i>dotted</i>) the application of adenosine. Note that adenosine increased the intervals of the mEPSC (decreased mEPSC frequency, p<0.001, Kolmogorov-Smirnov test). <b>D,</b> Cumulative amplitude distribution from the same cell before (<i>solid</i>) and during (<i>dotted</i>) the application of adenosine (p = 0.08, Kolmogorov-Smirnov test). <b>E,</b> Summarized data for adenosine-induced reduction of mEPSC frequency (n = 11, paired t-test). <b>F,</b> Adenosine failed to alter significantly mEPSC amplitudes (n = 11, paired t-test).