Involvment of GPER/eNOS signaling in the regulation of HIF-1α and CTGF expression.

<p>(A) Evaluation of HIF-1α and CTGF mRNA expression in WKY left ventricular tissues perfused with vehicle (-), 1 nmol/L G-1 alone (2 h) and in combination with 100 nmol/L G15 (1 h) or 10 µmol/L L-NIO (1 h), as evaluated by Real Time PCR and normalization to 18S expression. Bars represent the mean±SD of 5 experiments for each group. (○), (•) p<0.05. (B) Evaluation of HIF-1α and CTGF protein expression in WKY and SHR ventricular tissues treated with vehicle (-) or 1 nmol/L G-1 (2 h). Protein expressions were normalized to β-tubulin. Percentage changes were evaluated as mean±SD of 5 experiments for each group. (○), (•) p<0.05.