HIF-1α and CTGF expression in hypoxic cardiac preparations.

<p>(A) Evaluation of HIF-1α, GPER and CTGF mRNA expression by real time PCR in normoxic and hypoxic (1 h exposure to 40% pO₂ levels) WKY left rat ventricle after normalization to 18S expression. Bars represent the mean±SD of 5 experiments for each group. (○), (•), (□) p<0.05 for the expression of hypoxic vs normoxic preparations. (B) Representative immunoblots showing HIF-1α, GPER and CTGF protein expression in normoxic and hypoxic (1 h exposure to 40% pO₂ levels) male WKY rat left ventricle. Protein expressions were normalized to β-tubulin, percentage changes were evaluated as mean±SD of 5 experiments for each group. (○), (•), (□) p<0.05 for the expression of hypoxic vs normoxic preparations.