Detection of HIF-1α-dependent genes expression in whole hearts after H/R <i>in vivo.</i>

<p>Graphs show selected HIF-1α-dependent genes expression in whole hearts in control (C) and at different time points (3 h, 6 h, and 9 h) after H/R. (a) <i>Hmox1</i> mRNA expression exhibited a progressive increase and, at the 9 h time point, <i>hmox1</i> was ∼8-fold higher than in C. (b) <i>Vegfaa</i> mRNA increased and achieved its peak 6 h after H/R. (c) <i>Epo</i> mRNA exhibited a peak increase at 3 h which was ∼1.7-fold higher than in C but failed to achieve statistical significance. (n = 6; * <i>p</i><0.05 and *** <i>p</i><0.001 <i>vs.</i> C).