Med18/Mediator is required for Rrp6/Exosome-dependent H3K9 methylation at the pericentromere.

<p>(A) Silencing assay at the pericentromere. Shown are the results of serial dilutions of the indicated strains spotted onto N/S, 5-FOA and Low Ade media to assay <i>ura4⁺</i> and <i>ade6</i> expression. (B) ChIP analysis of H3K9me (left panel) and Swi6 (right panel) at <i>dh</i> repeats or <i>imr1L::ura4⁺</i> relative to <i>act1</i> or a tRNA genes (<i>trnaasn.05</i>), respectively. Error bars show the standard error of the mean (n = 3). (C) ChIP analysis of H3K9me (left panel) and Swi6 (right panel) at the <i>dh</i> repeats relative to <i>act1</i> or a gene-free region, respectively. Error bars show the standard error of the mean (n = 3). (D) ChIP analysis of Rrp6-13myc at <i>dh</i> repeats (left panel), <i>act1</i> (middle panel) and <i>fbp1</i> (right panel). Enrichment relative to the input whole cell extract (WCE) in the indicated strains are shown. Error bars represent the standard error of the mean (n = 3).