Cwf14 is required for pericentric heterochromatin assembly through the RNAi pathway.

<p>(A, E, F, and G) Serial dilution analyses to measure the expression of reporter genes and sensitivity to TBZ. (B and C) ChIP analyses of Pol II (Rpb1), H3K9me2, and Swi6 levels at pericentric <i>dh</i> repeats and <i>otr::ura4⁺</i>. Pol II ChIP was normalized to <i>act1</i> gene, and H3K9me2 and Swi6 ChIP was normalized to <i>act1</i> promoter. Error bars represent standard deviation of three experiments. (D) Northern blot analyses of siRNAs derived from pericentric <i>dh</i> repeats.