Loss of Suv39h enzymes affect H3K56me3.
- Antonia P. M. Jack (381337)
- Silva Bussemer (381338)
- Matthias Hahn (209009)
- Sebastian Pünzeler (381339)
- Martha Snyder (381340)
- Michael Wells (381341)
- Gyorgyi Csankovszki (5654086)
- Irina Solovei (85)
- Gunnar Schotta (116394)
- Sandra B. Hake (165089)
Publication date
February 2013
DOIAbstract
<p>IF microscopy of wild type (WT), Suv39h double-null (Suv39h DKO) and Suv4-20h double-null (Suv4-20h DKO) MEF cells using various H3K56 (A) and H3K9 (B) methyl-specific antibodies (Ab-Cy3, red) and DAPI (DNA, blue). Scale bar = 5 µm. (C) Immunoblots using acid extracted histones from HeLa Kyoto (positive control), wild type MEF, Suv39h DKO and Suv4-20h DKO cells. Blots were incubated with αH3K56me3 (left, top) or αH3K9me3 (right, top) antibodies, respectively. Blots shown at the bottom were incubated with αH4 to ensure equal loading.</p
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