Effect of <i>LmUAP1</i> and <i>LmUAP2</i> dsRNA on <i>UAP</i> transcript levels in <i>L. migratoria</i> by RT-qPCR.

<p>The fifth-instar nymphs on day 3 were injected with ds<i>GFP</i>, ds<i>LmUAP1</i> or ds<i>LmUAP2</i>, respectively. The silencing efficiency of the two <i>LmUAP</i> genes was evaluated at 24 h and 48 h after the injection of dsRNA as shown in (A) and (B). The genes encoding β-actin and GAPDH were used as internal controls. Data are expressed as means ± SD of three biological replications. ** Shows significant differences between the nymphs injected with ds<i>LmUAP1</i> or ds<i>LmUAP2</i> and those injected with ds<i>GFP</i> (<i>P</i><0.01, <i>t</i>- test); NS indicates no significant difference between the two groups.