The relative expression levels of four CarE genes in different developmental stages of <i>L. migratoria</i> as evaluated by RT-qPCR.

<p>The mRNA level in each of the samples, including eggs (EGG), first-instar nymphs on day1, 3 and 5 (N1D1, N1D3 and N1D5), second-instar nymphs on day1, 3 and 5 (N2D1, N2D3 and N2D5), third-instar nymphs on day1, 3 and 5(N3D1, N3D3 and N3D5), fourth-instar nymphs on day1, 3 and 5 (N4D1, N4D3 and N4D5), fifth-instar nymphs on day1, 3 and 5 (N5D1, N5D3 and N5D5) and adults on day1, 3 and 5 (AD1, AD3 and AD5), was normalized by β-actin as a reference gene. Results are mean and standard errors (SE) of three biological replications (n = 3), each with three technical replications. Different letters above the vertical bars indicate significant differences among the samples, based on Fisher's LSD multiple comparison test (<i>P</i><0.05).