Reduction in the normalized abundance of <i>V-ATPase D</i> and <i>V-ATPase B</i> transcripts in <i>Peregrinus maidis</i> by oral delivery of dsRNA.

<p>Third instar planthoppers were allowed to feed on dsRNA of <i>V-ATPase D,</i> dsRNA of <i>V-ATPase B</i> or dsRNA of GFP as a negative control treatment for up to six days. Insects were harvested two, four, and six days after feeding on dsRNA for real-time quantitative reverse transcriptase-PCR analysis of gene knockdown. The abundance of <i>V-ATPase D</i> (<b>A</b>) and <i>V-ATPase B</i> (<b>B</b>) transcripts was normalized to <i>P. maidis</i> ribosomal L10 transcripts. Each bar represents the mean and standard error of the mean of n = three experimental replicates each consisting of a group of three insects. The asterisk (*) indicates that treatment means differ significantly (<i>P</i><0.05) at a given time-point.