Time-dependent Aβ aggregation.

<p>(A) 30 nM QDAβ and 30 µM Aβ₄₂ were incubated in a 1536-well plate at 37 ^°C, and observed over time by an inverted fluorescence microscope using a 4x objective. All images show the same field of a well. (B) Variations of fluorescence intensities of 10,000 pixels (100 × 100 pixel) in the center region of micrographs were estimated as SD values, the mean values were plotted against incubation time periods. Error bars represent ±SDs of the mean values of fluorescence intensities (n=3 separate experiments).