The hIAPP aggregation behavior under highly crowded conditions monitored by ThT fluorescence spectroscopy.

<p>Aggregation time courses of hIAPP (10 µM) in solutions using (<b>A</b>) Ficoll, (<b>B</b>) dextran, (<b>C</b>) BSA and (<b>D</b>) lysozyme at concentrations of 10–40% (w/v) as crowding reagents. Left panels show absolute ThT fluorescence intensities, whereas the corresponding intensity normalized data are presented in the right panels. Data points are mean values (errors bars indicate standard deviations) of ≥6 experiments.