MiR-130b inhibited the growth of pancreatic cancer cells <i>in vitro</i> and <i>in vivo</i>.

<p>(A) The expression level of miR-130b was tested for 48 h in pancreatic cancer cells transfected with miR-130b mimics (miR-130b), anti-miR-130b and their respective NCs (50 nM) by qRT-PCR. (B) The effect of transient transfection of miR-130b or anti-miR-130b (50 nM) for 48 h was examined on the growth of PANC-1 and ASPC-1 cells by MTT assay. (C) The miR-130b inhibited the tumourigenicity in the nude mice xenograft model. The LV-miR-130b or LV-NC was transfected into PANC-1 cells in the presence of the virus at a multiplicity of infection (MOI) of 20. The infected PANC-1 cells were then subcutaneously injected into the nude mice. The tumor growth curves and the photographs of the excised tumors at 32 days post implantation are shown as indicated. Data are represented as the mean±SD in 10 mice. (D) The miR-130b expression levels were analyzed in excised tumors by qRT-PCR and were normalized to that of the endogenous control (U6 RNA). Data are shown as the mean±SD in 10 mice. *. P<0.05; **. P<0.01 as compared to the control.