STAT3 is the direct target of miR-130b in PANC-1 cells.

<p>(<b>A</b>) (Top panel) The human STAT3 3′UTR fragment containing wild-type or mutated miR-130b–binding sequence. (Bottom panel) The miR-130b and the miR-130b-binding site in the 3′UTR of STAT3. (<b>B</b>) Luciferase reporter assay with cotransfection of wild-type or mutant 3′UTR (100 ng) and miR-130b or miR-NC (50 nM) in PANC-1 cells. Firefly luciferase activity of each sample was normalized against Renilla luciferase activity. (<b>C</b>) The effects of miR-130b or anti-miR-130b on the expression of endogenous STAT3. QRT-PCR (left panel) and western blot (right panel) were used for monitoring the STAT3 expression in PANC-1 cells 48 h after the transfection with miR-130b or anti-miR-130b (50 nM). All data from three separate experiments are presented as mean±SD. *. P<0.05; **. P<0.01.