<p>DC were identified as in Figure 1. (A) Mean fluorescence intensity (MFI) of cell surface molecules on young and older B6 DCreg following culture. Data are mean ± SEM. N = 4-5 mice/group. (B) Young and older B6 DCreg culture supernatants were assessed for production of cytokines by ELISA. Data are mean ± SEM and represent 3 independent experiments.</p
<p>Young (n = 16) and middle-aged mice (n = 16) were injected with saline or 3 m/kg LPS and peritone...
<p>Freshly isolated splenocytes from (A) 8 week or (B) 8 month old female B6 or B6.NZBc1c13 (c1c13) ...
<p>Expression of the activation markers (A) B7.2 and MHCII was quantified by mean fluorescent intens...
<p>(A and B) Young and old mice were intravenously injected with 100 μg polyU/DO. Eighteen hours lat...
<p>A) The DC were generated from DBA/1J mice and cultured for 18 h with LPS, Zymosan or CpG in prese...
<p>Young and older BMT mice were treated with GFP<sup>+</sup> DCreg. DC populations were gated as de...
<p><i>A</i>, <i>B</i>, <i>C</i> and <i>D</i>. Following DC maturation, supernatant culture was colle...
<p>Cells were harvested from individual mice of each strain and cultured with CD3CD28 stimulation be...
<p>A) Evaluation of the Class II high populations of neonatal (7 days old) vs. adult mice shows diff...
<p>DC subsets were isolated from spleens of BCG infected (i.v.) mice at day 21 post-immunization as ...
<p>Immature mouse dendritic cells were stimulated with either recombinant mouse CD40L, or purified r...
<p>DCs (CD3-negative, CD19-negative, CD11c-positive, MHCII-positive) from CD11c-<i>Ifnar1</i><sup>-/...
<p>DCs from B6 mice were co-cultured with P29mtB6 or P29mtSAMP1 cybrids; the supernatant was harvest...
<p>BMDC from 8-12 week-old mice were expanded with FLT3L and then cultured in the presence or absenc...
<p>DCs were sorted from B6 mice and cultured at 5000 DCs per well in 96-well plates with (CpG or Pol...
<p>Young (n = 16) and middle-aged mice (n = 16) were injected with saline or 3 m/kg LPS and peritone...
<p>Freshly isolated splenocytes from (A) 8 week or (B) 8 month old female B6 or B6.NZBc1c13 (c1c13) ...
<p>Expression of the activation markers (A) B7.2 and MHCII was quantified by mean fluorescent intens...
<p>(A and B) Young and old mice were intravenously injected with 100 μg polyU/DO. Eighteen hours lat...
<p>A) The DC were generated from DBA/1J mice and cultured for 18 h with LPS, Zymosan or CpG in prese...
<p>Young and older BMT mice were treated with GFP<sup>+</sup> DCreg. DC populations were gated as de...
<p><i>A</i>, <i>B</i>, <i>C</i> and <i>D</i>. Following DC maturation, supernatant culture was colle...
<p>Cells were harvested from individual mice of each strain and cultured with CD3CD28 stimulation be...
<p>A) Evaluation of the Class II high populations of neonatal (7 days old) vs. adult mice shows diff...
<p>DC subsets were isolated from spleens of BCG infected (i.v.) mice at day 21 post-immunization as ...
<p>Immature mouse dendritic cells were stimulated with either recombinant mouse CD40L, or purified r...
<p>DCs (CD3-negative, CD19-negative, CD11c-positive, MHCII-positive) from CD11c-<i>Ifnar1</i><sup>-/...
<p>DCs from B6 mice were co-cultured with P29mtB6 or P29mtSAMP1 cybrids; the supernatant was harvest...
<p>BMDC from 8-12 week-old mice were expanded with FLT3L and then cultured in the presence or absenc...
<p>DCs were sorted from B6 mice and cultured at 5000 DCs per well in 96-well plates with (CpG or Pol...
<p>Young (n = 16) and middle-aged mice (n = 16) were injected with saline or 3 m/kg LPS and peritone...
<p>Freshly isolated splenocytes from (A) 8 week or (B) 8 month old female B6 or B6.NZBc1c13 (c1c13) ...
<p>Expression of the activation markers (A) B7.2 and MHCII was quantified by mean fluorescent intens...