Post-PEMF apoptosis determination by impedance flow cytometry (IFC) at 0.5 MHz.

<p>(<b>A</b>) Dot plots generated from MCF7 cell exposed to 2, 3 or 5 mT amplitude PEMFs for 60 minutes per day for three days. The histograms above and to the right of each dot plot show the apoptotic cell subpopulation shaded in black. MCF-7 cancer cells treated with 3 mT PEMFs exhibited the greatest separation between viable (right) and non-viable (left) cell populations as well as a higher overall percentage of dead cells. (<b>B</b>) Viability of MCF7 cells after exposure to 3 mT (20 Hz) for 30, 60 or 90 minutes per day for three days. (<b>C</b>) Percentage of MCF7 apoptotic cells detected by IFC in response to 2, 3 or 5 mT PEMFs normalized to its respective control. Each value represents the average of 4 independent experiments (1 replicate/experiment, n = 4) (± SD); P values, left to right: 0.4818, 0.0004552 and 0.1818. (<b>D</b>) Percentage of MCF7 dead cells in each treated sample normalized to its respective control in response to 30, 60 or 90 minutes exposures to PEMFs. Each value represents the average of 4 independent experiments (1 replicate/experiment, n = 4) (± SD). P-values, left to right: 0.1905, 0.0004552 and 0.3929. (<b>E</b>) MCF10 cells treated with PEMFs (3 mT, 20 Hz) for 60 minutes/day for three days. The dot plots shown were generated from cells of the same experimental date and are representative of cells responses observed in all of the independent experiments with identical conditions. Two different replicates obtained from 2 independents experiments were chosen for the 3 mT, 60 minute condition for figure <b>A</b> and <b>B.</b> Also see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0072944#pone.0072944.s008" target="_blank">Figure S8</a> for the spread of individual measurements.