Determination of morphological changes of tumor bearing EAC cells and percentage of apoptosis by fluorescence microscopy and flowcytometry, respectively.

<p>(3A) Tumor-bearing untreated control group (Group II) versus (3B) EAF treated, tumor-bearing group (Group III); arrows indicate apoptotic features, including condensed chromatin, apoptotic bodies, plasma membrane blebbing, and nuclear fragmentation. (3C) EAC cells were treated with EAF <i>in vitro</i> for different time intervals and percentage of apoptotic cells of total cells was analyzed by flow cytometry. Data are shown as means of 3 independent experiments (n = 3).