Endoret treatment decreased neurodegeneration in the brain of APP/PS1 mice.

<p>A. Representative photomicrographs showing degenerating neurons by Fluoro-Jade B staining in the cerebral cortex (Cx), and hippocampus (Hip) of 6 month-old APP/PS1 mice treated with vehicle and Endoret. Scale bar=20µm. Densitometric analysis of the number (left histogram) and size (in % right histogram) of Fluoro-Jade B-positive plaques in the cerebral frontal cortex (B) and the hippocampus (C) of APP/PS1 mice treated with vehicle and Endoret. D. Representative immunoblots of caspase-3 and synaptic proteins extracted from hippocampal synaptosome preparations. Densitometric quantification of changes in gray values expressed as mean ± SEM (vehicle-treated group, or control, is indicated as 100%). (D) Synaptophysin (green) in combination with DAPI-staining nuclei (blue), and (F) synapsin (red) in combination with bIII-Tubulin (green) labeling in hippocampus of APP/PS1 mice treated with vehicle and PRGF. Quantification of the effect of Endoret administration on synaptophysin (E) and synapin (G) immunoreactivity in hippocampus of APP/PS1 mice. n= 9 mice per group. Data show mean ± SEM; *p<0.05, **p<0.01 vs APP/PS1 + vehicle. Scale bar = 20µm.