Effect of microtubule and actin cytoskeleton on the centrosomal co-localization of fodrin and γ-tubulin.

<p>A. IMR32 cells were treated with Nocodazole (3.3 µM) for 2 hrs and then stained with α-fodrin (red), γ-tubulin (green) and α- tubulin (cyan). (B) IMR32 cells were treated with Cytochalasin B (1 µM) for 1 hr and then stained with either α-fodrin (red) and γ-tubulin (green) or with α-fodrin (red) and β actin (green). Control cell panels for γ-tubulin and fodrin staining have been reproduced from 7A as the experiments were done together. Co-localization is indicated by arrowheads and the absence of co-localization is shown by arrows. Primary antibodies were used in 1∶200 dilution and secondary antibodies were in 1∶500 dilution.