Effect of inhibiting microtubule dynamics on ch-TOG localisation.

<p>(<b>A, B</b>) ch-TOG-GFP (red) was expressed in HeLa cells expressing RFP-α-tubulin (green) and a low concentration of taxol (500 nM) was added. The living cells were observed by TIRF. The boxed area in (A) is enlarged in (B). Scale bars; 10 µm (A), 2 µm (B). (<b>C, D</b>) A HeLa cell clone expressing GFP-α-tubulin (1E10, green) was fixed and stained for endogenous ch-TOG using a secondary antibody conjugated with RhoX (red), and observed by SIM. The boxed area in (C) is enlarged in (D). Scale bars; 10 µm (A), 2 µm (B).