Quantitation of γ-tubulin and fodrin in interphase and mitotic cells: IMR 32 cells from the above experiment were analyzed for γ-tubulin or fodrin content in green chanel and red channel respectively.

<p>The ROI was selected to cover the whole centrosome in different cells and the total fluorescence intensity (pixels) was obtained by multiplying the mean intensity with centrosome area. The background was selected similarily from the cytoplasmic area and subtracted from the centrosomal intensity. The average of thus calculated intensities was determined from 21 centrosomes and plotted. Standard deviation was calculated from three experiments.