Phenotype of matured DCs.

<p>DCs matured as described in the methods section were labeled with anti-CD11c and either anti-CD86, anti-H2k^b (MHC I), anti-I-A/I-E (MHC II), anti-CD40, anti-CD70, anti-PDL-1 or anti-PDL2 and analyzed by flow cytometry gating on CD11c+ cells. Black histograms represent untreated DCs, DCs treated with 10ng/ml TNF-α are in blue, 1µM CpG in green, 1µg/ml CD40L in red and 0.1 µg/ml LPS in orange. Note that CD40L stimulated cells do not express surface CD40, likely due to receptor internalization.