Representative flow cytometry analysis of DC culture.

<p>A) DC culture development. Lymphocytes (CD3+) and death cells (IP positive) were discarded. DCs were backgated using a FSC-A/SSC-A dot plot. Subsequently, CD11c and CD11b expression were assessed in DC population using CD11c APC/CD11bPE dot plot. D0, D3, D5 and D8 = day 0, 3, 5 and 8 respectively B) CD40 expression of DC with and without LPS stimuli and transfected with Chol-siRNA or SC siRNA were analyzed by flow cytometry (MFI). *p<0.05 vs control and scrambled. C) Representative histograms for each group of treatment. D) Representative histograms of siRNA internalization analyzed by flow citometry (MFI). E) DC cytokine secretion after LPS stimulus and Chol-siRNA effect. F) Chol-siRNA Cy5.5 internalization in DCs is shown at time 0 and after 45 min of incubation (upper left and right photomicrograph x630, respectively). Middle and bottom photomicrographs: Z-scan image of live DC cells where we can observe siRNA cytoplasm localization and confocal projections of the cell to the xz and zy planes (sideviews). Data are expressed as mean± SEM of four separate experiments.