Phenotype analysis of different DCs.

<p>(A) FACS histograms showed the expression of cell surface markers on iDCs, which expressed low levels of CD80, MHCII, OX62, CD86 and CD11b/c. OX62 expressed much higher than other makers, because OX62 is an important marker of rat DCs. (B) LPS- stimulated mature DCs (mDCs) showed about equally up-regulated levels of all markers. Except CD86, three makers showed above 90 percent expression. (C) MSC-DC-LPS-activated DCs in co-culture expressed lower levels of the four markers compared with mDCs, but the expression was slightly higher when compared with iDCs. This result suggests that MSCs inhibit DCs phenotype expression (MSC:DC = 1∶10). Data represent mean fluorescence intensity for the surface density of markers. (D) Quantification by image analysis of the positive cells. The numbers in the histograms indicate the mean fluorescence of each DCs markers. *P<0.05(mDC group versus iDC group), #P<0.05(MSC-DC group versus mDC group).