The new and special phenotype of DC cells in AT of ND and HFD mice.

<p>(A) The DCs that infiltrated into AT are immature DCs. Multicolor FCM analyses were performed on single cell suspensions that were stained with specific mAb against FITC-CD11c and PE-CD40, CD80, CD86 MHCI and MHCII, cells were gated on the CD11c population. Numbers in histograms represent the geometric mean fluorescence of the gated cells. Open histograms indicate isotype control. (B) The ATDCs of HFD mice have the relatively stable phenotype with or without LPS stimulation. The ATDCs were incubated with or without LPS (100 ng/ml) stimulation at 37°C for 12 hours and stained with specific mAb against FITC-CD11c and PE-CD40, CD80, CD86, MHCI and MHCII. Numbers in histograms represent the geometric mean fluorescence of the gated CD11c cells. (C) The ATDCs of HFD mice have the relatively phagocytosis ability with or without LPS stimulation. The ATDCs were incubated with or without LPS stimulation and HcRed-expressed <i>E.coli</i> at 37°C for 4 hours. Data are representative of three independent experiments.