Pharmacological confirmation of RNAi effect targeting at 5HTR_B.

<p>Effect of injections of 5HT, 5,7-DHT and luzindole on photoperiodism. (A) Diapause pupae were injected either with 5 µl water and 5 µl DMSO (Mock injection) or 5 pmoles Luzindole plus 5 pmoles 5HT in the same volume of solvent and placed under LD: 16:8 at 25°C. Cumulatively 5% adults emerged in 40 days after injection. Cont.: untreated. M: injection with distilled water and DMSO. Luzindole +5HT: luzindole and 5HT co-injected. (B) Diapause pupae were injected with 5,7-DHT at three doses and thereafter the pupae were kept under LD 12:12 at 25°C. Cont.: untreated. M: mock injection with 10 µl distilled water. 5,7-DHT: injected with 5,7-DHT dissolved in the same volume as in the mock. 5 percentage points, 30 percentage points and 100 percentage points adult emergence was observed in 40 days. Asterisks indicate significant difference from control by Kaplan-Meier. ** <i>p</i><0.01.